Semi-Blind Spatially-Variant Deconvolution in Optical Microscopy with Local Point Spread Function Estimation By Use Of Convolutional Neural Networks

03/20/2018

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by   Adrian Shajkofci, et al.

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We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy algorithm. To find the local PSF map in a computationally tractable way, we train a convolutional neural network to perform regression of an optical parametric model on synthetically blurred image patches. We deconvolved both synthetic and experimentally-acquired data, and achieved an improvement of image SNR of 1.00 dB on average, compared to other deconvolution algorithms.