-
All-in-Focus Iris Camera With a Great Capture Volume
Imaging volume of an iris recognition system has been restricting the th...
read it
-
Axially-shifted pattern illumination for macroscale turbidity suppression and virtual volumetric confocal imaging without axial scanning
Structured illumination has been widely used for optical sectioning and ...
read it
-
Rapid Whole Slide Imaging via Learning-based Two-shot Virtual Autofocusing
Whole slide imaging (WSI) is an emerging technology for digital patholog...
read it
-
Towards a Magnetically Actuated Laser Scanner for Endoscopic Microsurgeries
This article presents the design and assembly of a novel magnetically ac...
read it
-
Compressive sensing on diverse STEM scans: real-time feedback, low-dose and dynamic range
Scanning Transmission Electron Microscopy (STEM) has become the main sta...
read it
-
Recurrent neural network-based volumetric fluorescence microscopy
Volumetric imaging of samples using fluorescence microscopy plays an imp...
read it
-
Randomized Multiresolution Scanning in Focal and Fast E/MEG Sensing of Brain Activity with a Variable Depth
We focus on electromagnetoencephalography imaging of the neural activity...
read it
Rapid focus map surveying for whole slide imaging with continues sample motion
Whole slide imaging (WSI) has recently been cleared for primary diagnosis in the US. A critical challenge of WSI is to perform accurate focusing in high speed. Traditional systems create a focus map prior to scanning. For each focus point on the map, sample needs to be static in the x-y plane and axial scanning is needed to maximize the contrast. Here we report a novel focus map surveying method for WSI. The reported method requires no axial scanning, no additional camera and lens, works for stained and transparent samples, and allows continuous sample motion in the surveying process. It can be used for both brightfield and fluorescence WSI. By using a 20X, 0.75 NA objective lens, we demonstrate a mean focusing error of 0.08 microns in the static mode and 0.17 microns in the continuous motion mode. The reported method may provide a turnkey solution for most existing WSI systems for its simplicity, robustness, accuracy, and high-speed. It may also standardize the imaging performance of WSI systems for digital pathology and find other applications in high-content microscopy such as DNA sequencing and time-lapse live-cell imaging.
READ FULL TEXT
Comments
There are no comments yet.