Figure 1
a
H3K4 Methyl-proteoforms Model
Figure 2
[width = .99]HIquant_validation_2.pdf
Figure 3
[width = .42]H3K4.pdf
accurately infers stoichiometries and confidence intervals across PTM site occupancies of histone 3. (
a) Histone 3 peptides were quantified by SRM across 7 perturbations, and the fractional site occupancies for K4 methylation estimated by two methods: Estimates inferred by HIquant without using external standards are plotted against the corresponding estimates based on MasterMix external standards with known concentrations creech2015building. Each marker shape corresponds to the PTM site(s) shown in the legend; methylation is denoted with “me” and acetylation with “ac” followed by the number of methyl/acetyl groups. (b) The validation method from (a) was extended to another set of more complex fractional site occupancies on K9 methylation and K14 acetylation.
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