DeepAI AI Chat
Log In Sign Up

PhaseStain: Digital staining of label-free quantitative phase microscopy images using deep learning

by   Yair Rivenson, et al.

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images of the same samples that are histochemically stained. Through pairs of image data (QPI and the corresponding brightfield images, acquired after staining) we train a generative adversarial network (GAN) and demonstrate the effectiveness of this virtual staining approach using sections of human skin, kidney and liver tissue, matching the brightfield microscopy images of the same samples stained with Hematoxylin and Eosin, Jones' stain, and Masson's trichrome stain, respectively. This digital staining framework might further strengthen various uses of labelfree QPI techniques in pathology applications and biomedical research in general, by eliminating the need for chemical staining, reducing sample preparation related costs and saving time. Our results provide a powerful example of some of the unique opportunities created by data driven image transformations enabled by deep learning.


page 16

page 17

page 18

page 19

page 20


Single-shot autofocusing of microscopy images using deep learning

We demonstrate a deep learning-based offline autofocusing method, termed...

Deep Learning Models for Digital Pathology

Histopathology images; microscopy images of stained tissue biopsies cont...

Virtual stain transfer in histology via cascaded deep neural networks

Pathological diagnosis relies on the visual inspection of histologically...

Generation and Simulation of Yeast Microscopy Imagery with Deep Learning

Time-lapse fluorescence microscopy (TLFM) is an important and powerful t...

Virtual organelle self-coding for fluorescence imaging via adversarial learning

Fluorescence microscopy plays a vital role in understanding the subcellu...

Detection of Parasitic Eggs from Microscopy Images and the emergence of a new dataset

Automatic detection of parasitic eggs in microscopy images has the poten...

Label-free virtual HER2 immunohistochemical staining of breast tissue using deep learning

The immunohistochemical (IHC) staining of the human epidermal growth fac...