Observation of dynamics inside an unlabeled live cell using bright-field photon microscopy: Evaluation of organelles' trajectories

12/13/2016
by   Renata Rychtarikova, et al.
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This article presents an algorithm for the evaluation of organelles' movements inside of an unmodified live cell. We used a time-lapse image series obtained using wide-field bright-field photon transmission microscopy as an algorithm input. The benefit of the algorithm is the application of the Rényi information entropy, namely a variable called a point information gain, which enables to highlight the borders of the intracellular organelles and to localize the organelles' centers of mass with the precision of one pixel.

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