Effects of Purine Bases and Nucleosides on the Survival of Cortical Rat Astrocytes in vitro

06/05/2020
by   Daniel Cebo, et al.
0

Purines are responsible for the regulation of many physiological and pathological processes in the central nervous system including the development and remodeling of tissues after trauma, stress, ischemia, and neurodegenerative diseases. The extracellular concentration of purines significantly increases under conditions of stress. We investigated the effects of purine nucleosides and nucleobases on primary cultures of Wistar rat astrocytes not only under physiological conditions, but also after deprivation of energy depots by using iodoacetic acid and assessed whether the investigated molecules exhibited cytoprotective or cytotoxic effects. An MTT test was used to evaluate cell viability, whereas flow cytometry analysis was employed to evaluate the presence of free oxygen species. Survival of astrocytes (as evidenced by the MTT test) decreased on treatment with 500 µM iodoacetic acid for 40 min (to 58.4% compared with untreated controls). The cytotoxic effect of iodoacetic acid was partially reversed by co-treatment with either 200 µM adenosine (to 80.3% of control) or 200 µM guanosine (to 83.3% of control), whereas the application of inosine or adenine had no effect. Iodoacetic acid alone did not increase the production of free oxygen radicals, but the induction of oxidative stress was observed during the concurrent incubation of astrocytes with 200 µM adenosine and 500 µM iodoacetic acid. We conclude that, under energy deprivation, adenosine and guanosine stimulate the survival of astrocytes by means of the recovery of intracellular ATP.

READ FULL TEXT
research
04/16/2021

Causal Mediation Analysis for Longitudinal Mediators and Survival Outcomes

Causal mediation analysis studies how the treatment effect of an exposur...
research
07/27/2018

Survival of the Fittest Group: Factorial Analyses of Treatment Effects under Independent Right-Censoring

This paper introduces new effect parameters for factorial survival desig...
research
02/03/2023

Factorial survival analysis for treatment effects under dependent censoring

Factorial analyses offer a powerful nonparametric means to detect main o...
research
12/31/2020

Effects of End Restraints on Thermo-Mechanical Response of Energy Piles

Currently, soil structure interaction in energy piles has not been under...
research
04/08/2023

Interpretable machine learning-accelerated seed treatment by nanomaterials for environmental stress alleviation

Crops are constantly challenged by different environmental conditions. S...

Please sign up or login with your details

Forgot password? Click here to reset